Newcastle striker Loic Remy will sit out the derby clash with Sunderland after failing in a bid to have his sending-off at Norwich overturned.
The Magpies lodged an appeal against the 27-year-old's dismissal along with Canaries midfielder Bradley Johnson after the pair clashed late in Tuesday night's 0-0 Barclays Premier League draw at Carrow Road.
However, while Johnson's own effort to clear his name was upheld, Remy's fell on deaf ears and he will now miss the league games against the Black Cats, Chelsea and Tottenham.
A Football Association statement said: "An independent regulatory commission heard two claims of wrongful dismissal today both in relation to the Norwich City versus Newcastle United game on Tuesday.
"The claim involving Norwich's Bradley Johnson was upheld, meaning his three-match suspension is withdrawn.
"The claim involving Newcastle's Loic Remy was dismissed, therefore his three-match suspension remains with immediate effect.
"Both players had been dismissed for violent conduct."
Although the news was not unexpected, it will come as a blow to manager Alan Pardew, who is also likely to be without injured duo Yoan Gouffran and Papiss Cisse as he attempts to avoid a third successive derby defeat.
In the circumstances, he has challenged new signing Luuk de Jong to make himself an instant hero on Tyneside.
The 23-year-old striker completed a loan move for the rest of the season to St James' Park on Wednesday, and will go straight into the squad for the derby.
De Jong has not started a single game for Borussia Monchengladbach this season and his last goal for the club came in April, but Pardew has told him to be ready to make a swift impact.
The 52-year-old said: "I said to him today, 'You could become an instant hero if you play or you get a patch on the pitch in terms of a substitute's appearance'.
"That must be his immediate dream. It certainly would be mine if I was a striker."
De Jong's arrival in the north-east came some 18 months after the Magpies first tried to sign him, and Pardew is hoping it will be worth the wait after his spell in the Bundesliga proved a difficult one.
He said: "I'm pleased in so much that he's a player we have had our eye on for a while.
"He was someone we couldn't quite reach in his last transfer move in terms of the finance, but that hasn't worked out as perfectly as perhaps they both hoped, the team and Luuk, and he's ended up here.
"But he has done well today and we are looking forward to his inclusion in the squad. He's a big presence of a player, he's a goalscorer and if there's a criticism you would aim at us, maybe we could do with scoring a few more goals."
The Holland Under-21 international has already signalled his readiness to start the derby if required, but Pardew has hinted that that is unlikely despite his relative lack of options.
He said: "Well, he hasn't had much game action and this is the Premier League, so I would suggest that I might be a better judge than he is.
"But I like his confidence and he has trained very well today, so I am very pleased with him."
Pardew is hoping against hope that De Jong is not the only new arrival ahead of Friday night's transfer deadline, with the club's bid for Lyon midfielder Clement Grenier still on the table as they look for a replacement for Yohan Cabaye.
The manager insisted as it became obvious that Cabaye would get his move to Paris St Germain earlier this week that he would have to be replaced, and the clock is now ticking.
Asked if he was hopeful of landing Grenier, Pardew said: "I don't know. We have two days left and we will have to wait and see. I'm obviously hopeful and that's where I sit today."
Asked further if his hand would be weakened should no replacement arrive, he added: "Well, of course. You can't take a player out like Cabaye and say it's not going to weaken you. It is, unless we can replace him like for like.
"But whether we can do that, I don't know."